SrasV1, Main, Exploration, bibRecord, 002F41

Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model

Identifieur interne : 002F41 ( Main/Exploration ); précédent : 002F40; suivant : 002F42

Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model

Auteurs : HUI HU [République populaire de Chine] ; XIANQING HUANG [République populaire de Chine] ; LING TAO [République populaire de Chine] ; YI HUANG [République populaire de Chine] ; Bao-An Cui [République populaire de Chine] ; HANZHONG WANG [République populaire de Chine]

Source :

Vaccine [ 0264-410X ] ; 2009.

RBID : Pascal:09-0167255

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Immunogénicité, Nucléocapside, Vaccin génétique, Modèle animal, Protéine, Immunisation.

English descriptors

KwdEn :
- Animal model, Genetic vaccine, Immunization, Immunogenicity, Nucleocapsid, Protein, Severe acute respiratory syndrome virus.

Abstract

The development of strategies to augment the immunogenicity of DNA vaccines is critical for improving their clinical utility. One such strategy involves using the different immune routes with DNA vaccines. In the present study, the immunogenicity of SARS-CoV nucleocapsid DNA vaccine, induced by using the current routine vaccination routes (intramuscularly, by electroporation, or orally using live-attenuated Salmonella typhimurium), was compared in mouse model. The comparison between the three vaccination routes indicated that immunization intramuscularly induced a moderate T cell response and antibody response. Mice administrated by electroporation induced the highest antibody response among the three immunization groups and a mid-level of cellular response. In contrast, the orally DNA vaccine evoked vigorous T cell response and a weak antibody production. These results indicated that the distinct types of immune responses were generated by the different routes of DNA immunization. In addition, our results also show that the delivery of DNA vaccines by electroporation and orally using live-attenuated Salmonella in vivo is an effective method to increase the immune responses. Further studies could be carried out using a combination strategy of both oral and electroporation immunizations to stimulate higher cellular and humoral immune responses.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000223
to stream PascalFrancis, to step Curation: 000765
to stream PascalFrancis, to step Checkpoint: 000223
to stream Main, to step Merge: 003000
to stream Main, to step Curation: 002F41

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model</title>
<author><name sortKey="Hui Hu" sort="Hui Hu" uniqKey="Hui Hu" last="Hui Hu">HUI HU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Animal Science and Veterinary Medicine, Henan Agricultural University</s1>
<s2>Zhengzhou, Henan 450002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Zhengzhou, Henan 450002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xianqing Huang" sort="Xianqing Huang" uniqKey="Xianqing Huang" last="Xianqing Huang">XIANQING HUANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Animal Science and Veterinary Medicine, Henan Agricultural University</s1>
<s2>Zhengzhou, Henan 450002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Zhengzhou, Henan 450002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ling Tao" sort="Ling Tao" uniqKey="Ling Tao" last="Ling Tao">LING TAO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yi Huang" sort="Yi Huang" uniqKey="Yi Huang" last="Yi Huang">YI HUANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cui, Bao An" sort="Cui, Bao An" uniqKey="Cui B" first="Bao-An" last="Cui">Bao-An Cui</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Animal Science and Veterinary Medicine, Henan Agricultural University</s1>
<s2>Zhengzhou, Henan 450002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Zhengzhou, Henan 450002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">09-0167255</idno>
<date when="2009">2009</date>
<idno type="stanalyst">PASCAL 09-0167255 INIST</idno>
<idno type="RBID">Pascal:09-0167255</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000223</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000765</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000223</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000223</idno>
<idno type="wicri:doubleKey">0264-410X:2009:Hui Hu:comparative:analysis:of</idno>
<idno type="wicri:Area/Main/Merge">003000</idno>
<idno type="wicri:Area/Main/Curation">002F41</idno>
<idno type="wicri:Area/Main/Exploration">002F41</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model</title>
<author><name sortKey="Hui Hu" sort="Hui Hu" uniqKey="Hui Hu" last="Hui Hu">HUI HU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Animal Science and Veterinary Medicine, Henan Agricultural University</s1>
<s2>Zhengzhou, Henan 450002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Zhengzhou, Henan 450002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xianqing Huang" sort="Xianqing Huang" uniqKey="Xianqing Huang" last="Xianqing Huang">XIANQING HUANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Animal Science and Veterinary Medicine, Henan Agricultural University</s1>
<s2>Zhengzhou, Henan 450002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Zhengzhou, Henan 450002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ling Tao" sort="Ling Tao" uniqKey="Ling Tao" last="Ling Tao">LING TAO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yi Huang" sort="Yi Huang" uniqKey="Yi Huang" last="Yi Huang">YI HUANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cui, Bao An" sort="Cui, Bao An" uniqKey="Cui B" first="Bao-An" last="Cui">Bao-An Cui</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Animal Science and Veterinary Medicine, Henan Agricultural University</s1>
<s2>Zhengzhou, Henan 450002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Zhengzhou, Henan 450002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</s1>
<s2>Wuhan 430071</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal model</term>
<term>Genetic vaccine</term>
<term>Immunization</term>
<term>Immunogenicity</term>
<term>Nucleocapsid</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome virus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Immunogénicité</term>
<term>Nucléocapside</term>
<term>Vaccin génétique</term>
<term>Modèle animal</term>
<term>Protéine</term>
<term>Immunisation</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The development of strategies to augment the immunogenicity of DNA vaccines is critical for improving their clinical utility. One such strategy involves using the different immune routes with DNA vaccines. In the present study, the immunogenicity of SARS-CoV nucleocapsid DNA vaccine, induced by using the current routine vaccination routes (intramuscularly, by electroporation, or orally using live-attenuated Salmonella typhimurium), was compared in mouse model. The comparison between the three vaccination routes indicated that immunization intramuscularly induced a moderate T cell response and antibody response. Mice administrated by electroporation induced the highest antibody response among the three immunization groups and a mid-level of cellular response. In contrast, the orally DNA vaccine evoked vigorous T cell response and a weak antibody production. These results indicated that the distinct types of immune responses were generated by the different routes of DNA immunization. In addition, our results also show that the delivery of DNA vaccines by electroporation and orally using live-attenuated Salmonella in vivo is an effective method to increase the immune responses. Further studies could be carried out using a combination strategy of both oral and electroporation immunizations to stimulate higher cellular and humoral immune responses.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Hubei</li>
</region>
<settlement><li>Wuhan</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Hui Hu" sort="Hui Hu" uniqKey="Hui Hu" last="Hui Hu">HUI HU</name>
</region>
<name sortKey="Cui, Bao An" sort="Cui, Bao An" uniqKey="Cui B" first="Bao-An" last="Cui">Bao-An Cui</name>
<name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name>
<name sortKey="Hui Hu" sort="Hui Hu" uniqKey="Hui Hu" last="Hui Hu">HUI HU</name>
<name sortKey="Ling Tao" sort="Ling Tao" uniqKey="Ling Tao" last="Ling Tao">LING TAO</name>
<name sortKey="Xianqing Huang" sort="Xianqing Huang" uniqKey="Xianqing Huang" last="Xianqing Huang">XIANQING HUANG</name>
<name sortKey="Yi Huang" sort="Yi Huang" uniqKey="Yi Huang" last="Yi Huang">YI HUANG</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F41 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002F41 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:09-0167255
   |texte=   Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model

Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri